Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato

Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato

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Fusarium oxysporum Schlecht.f.sp.

radicis-lycopersici Jarvis & Schoemaker (FORL) is a saprophytic fungus, responsible for the fusarium crown and root rot disease quest fryer in tomato (Solanum lycopersicum L.).This is one of the most destructive pathogens of this species.

A new cleaved amplified polymorphic sequence (CAPS) marker C2-25 was developed for the detection of the dominant gene Frl, which confers tomato resistance to FORL.C2-25 was amplified from a conserved ortholog set II (COSII) sequence C2_At2g38025.The 5326058hx XapI-derived restriction product of 700 bp was informative for the identification of FORL resistant tomato genotypes and can be used as a diagnostic marker in tomato breeding programmes and hybrid seed production.